• Science Education

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    Share this...FacebookLinkedinGoogle+TwitterPrintemail We are transferring our contents directly to your inboxes. For this, you just have to feed your email ID and confirm the receipt of our email in your inbox. Future updates will automatically feed in your mails. 1

  • Image Duplication,  Quality research,  Science Education

    Larvae moving together for a photoshoot for another experiment

    Share this...FacebookLinkedinGoogle+TwitterPrintemailIf a researcher is doing some experiments with animal species, it is quite obvious that animal tends to move from one place to other. Movement of any animal species in a laboratory is difficult to manage. For this, we have to screw up the caps of culture tubes, put the tightened cotton plugs, clean the floors routinely and put an animal back if it comes out of its boundaries. Below we are discussing the possibilities, how a pair of larvae moved out of an experimental set to get a photoshoot for another set of conditions. Molecular biologist treats larvae with siRNA to check effect as lethality or as growth…

  • Quality research,  Science Education

    An author should explain more about the relevant experimental details

    Share this...FacebookLinkedinGoogle+TwitterPrintemailFrom a research article, we assume that author made everything clear and one could follow the research easily. This is the only reason, we made our research publicly available. But there are articles which led to the confusion to the researchers. The below-discussed article is among those ones. Here author used a gene to transform a model plant, but; • No cloning strategy (restriction sites, size) has been given for the gene;• Growth media compositions (YEM, root medium and other tissue culture media) are given in fine details (with elemental composition) for a model plant, unnecessarily.• For transgenics confirmation, both NPTII and gene-specific primer sequence and annealing temperature (Ta) is given…

  • Fabrication,  Quality research,  Science Education

    Southern or Photoshop! Use any for Transgenics Confirmation

    Share this...FacebookLinkedinGoogle+TwitterPrintemailWith Photoshop, we can do a lot of experimentation, like done here. Molecular characterization of transgenics is done to check the stable integration of the T-DNA cassettes. In two back to back papers authors did the experimentation to prove that transgenics are stable and contain the T-DNA sequence.  Two important sights are: Whenever we run an Agarose gel all the lanes are likely separated by equal distances, because of similar gaps between the comb teeth. However, in these pictures of the Southern blots, the gap between lanes is unequal, which is noticeable. This unequal distance led us to carefully investigate for the bands. Upon examination, we found that bands are created…

  • Quality research,  Science Education

    Does Over-expression of a gene leads to gene loss in rice?

    Share this...FacebookLinkedinGoogle+TwitterPrintemailBelow given picture is to confirm the presence of transgenes in rice transgenics. For over-expression studies, transgenics have been developed using GLYI gene of Brassica and GLYII gene of Rice. Upon transformation, it is obvious that WT Rice plants (controls) would show up a signal for presence of genomic GLYII gene. This is because, gene has been cloned from Rice and highly conserved among rice species. However, author didn’t detect presence of even a faint band in either PCR or Southern experiments. What could be the possible reason? We can discuss here. Full length article can be inquired at admin@vigilantscientist.com 9

  • Quality research,  Science Education

    Doing experiments unnecessarily and mis-interpreting data

    Share this...FacebookLinkedinGoogle+TwitterPrintemailBelow given picture is from Southern experiments of Rice T0 and T1 transgnics for very famous CRY genes transformation. Analysis of transgenics Here an unnecessary experiments has been done which does not add any value to the “PNAS” paper and “Bose Institute” and a mis-interpretation of data is given which is purely bases on assumpions. Please check and discuss with your friends to search for the issues highlighted. 8

  • Science Education

    Antar Dhundo- search for the differences

    Share this...FacebookLinkedinGoogle+TwitterPrintemail Here are the Northern blots (RNA hybridization experiments) to examine the expression of a gene under various salt concentration and different days interval (A-D lanes) with rRNA controls in E lane. In the lanes, we found differences at 3 spots in intense (dark) bands. It would be intersting if you can find these differences or more if any.  A response can be sent as comments, mail, chat like e.g. B50, D100 etc. (mentioning days interval and salt concentration). 1