• Fabrication,  Image Duplication

    Genetic/Gel Engineering

    Share this...FacebookLinkedinGoogle+TwitterPrintemailBelow is a marvelous example of gel fabrication. You can call it “gel engineering” instead of “genetic engineering”. In the picture, three PCR reactions for three genes and corresponding gels has been provided. But author had smartly made the engineering of gels to create this example. We identified a minimum of 4 spots where engineering has been done in these gels. We want that you should identify these spots and send us the replies at info@vigilantscientist.com or as comments. This will be the part of our quiz which holds a surprise gift for you. Fun and Learn 1

  • Fabrication

    Molecular Markers-Its easy to make and hide fabricated gels in a crowd of lanes

    Share this...FacebookLinkedinGoogle+TwitterPrintemailIn continuation, with yesterdays post we found articles from the same laboratory contain gels of molecular markers which are highly fabricated. Below is the gel picture from Plant Science Journal where SCoT molecular markers have been used to check the polymorphism among a germplasm. The experiment is to demarcate the toxic and non-toxic features. However, author smartly fabricated the gel after running to show the results. From the data, it is difficult to relate the findings to the toxic and non-toxic feature of plants. Hence the whole experimentation and article are of no use both to the science or society. These experiments are very harmful and their presence in database could mislead the followers.…

  • Fabrication,  Image Duplication

    In a gel, few well chose to double themselves. Why?

    Share this...FacebookLinkedinGoogle+TwitterPrintemailAfter a long vacation of Deepawali, we are with a very new gel picture, where author fabricated the gel lanes. From the gel, it is clear that the picture was available for 38 samples (1-38) and after run completion, it has been decided to make a copy of the selected lane (17-21) and paste it as different samples (39-43).   We sent the data to the authors for moderation and article link will be updated soon. Please share your comments on this data. 2

  • Fabrication,  Image Duplication,  Plagiarism,  Quality research

    A detailed story of the moving larvae …

    Share this...FacebookLinkedinGoogle+TwitterPrintemailIn our earlier post, we found that same larvae has been planned to move for another photograph. close examination of the photographs reveals that only a single photograph has been taken and second photograph has been used as cropped version. We mailed author  to explain this incidence, but did not get any replies. Hence we are providing a link to the article. http://10.1016/j.jinsphys.2008.12.005 In the article, author aimed to Silence acetylcholinesterase gene of Helicoverpa armigera by feeding siRNA supplemented in an artificial diet and reports that siRNA affects the larval growth and its life cycle. As we mentioned earlier, first photograph is to check the lethal dose of AChE-siRNA on larval growth. …

  • Fabrication,  Quality research,  Science Education

    Southern or Photoshop! Use any for Transgenics Confirmation

    Share this...FacebookLinkedinGoogle+TwitterPrintemailWith Photoshop, we can do a lot of experimentation, like done here. Molecular characterization of transgenics is done to check the stable integration of the T-DNA cassettes. In two back to back papers authors did the experimentation to prove that transgenics are stable and contain the T-DNA sequence.  Two important sights are: Whenever we run an Agarose gel all the lanes are likely separated by equal distances, because of similar gaps between the comb teeth. However, in these pictures of the Southern blots, the gap between lanes is unequal, which is noticeable. This unequal distance led us to carefully investigate for the bands. Upon examination, we found that bands are created…

  • Fabrication,  Image Duplication,  Plagiarism

    Twist, Flip, Shift; Ready to publish.

    Share this...FacebookLinkedinGoogle+TwitterPrintemailEarlier we identified that gene is missing in a PCR reaction and Southern experiment of the Rice transgenics. This is the content of an article under DOI (Could be enquired through mail at admin@vigilantscientist.com) Interaction with authors revealed that, a critical piece of information (about cloning and restriction digestion strategy in pCAMBIA, primer sequences for PCR and cloning, complete Southern blot figure) is missing in the article. Keeping it aside, we found that article is not carrying a single labeling error, but contains a smartly fabricated photograph which is very unique and new to our posts. In same article, author gave two different stress treatments i.e. Salt and Methylglyoxal (MG)…

  • Fabrication,  Image Duplication,  Plagiarism

    One Picture; Many Papers i.e. JNU & ICGEB

    Share this...FacebookLinkedinGoogle+TwitterPrintemailFrom the producer of our story Antar Dhundo, we found that there is not a single event for image manipulation in the article. The follow-up studies have the usage of the same photographs again and again. From the below-given panels (4 different panels) you can see how the same figures have been used in 3 different articles. Institutions of national reputation like JNU and ICGEB are enjoying huge funding to carry out these studies from DBT, DST, CSIR etc. http://10.1099/mic.0.019869-0http://10.1074/jbc.m110.111021http://10.4161/psb.6.5.15106 4

  • Fabrication,  Image Duplication,  Plagiarism

    Samanta Dhundo…. Find for the similars

    Share this...FacebookLinkedinGoogle+TwitterPrintemailDear Friends, Today’s story is exactly opposite the Antar Dhundo story. Here we have to find for the similars. Find for the similars Each lane contain different samples of glycine max (either roots or leaves under salt stress condition) and a semi-q-RT-PCR reaction was performed using different pairs of primers corresponding to the genes highlighted in front of the rows. In this figure a few (3-4) lanes of 7 samples each are similar. Article is produced from India and China in collaboration. Let’s see how many you can highlight. 7

  • Fabrication,  Publishing Error,  Quality research

    Article in press! Figure Altered! Published Differently. Reason for Antar Dhundo..

    Share this...FacebookLinkedinGoogle+TwitterPrintemailYou might have enjoyed our post “Antar Dhundo“, as it contains two panels 1 and 2, which are almost similar with a few differences, which we asked you to identify. You will be amazed to know that two panels are for “Northern blots” of RNA isolated from different samples harvested under two variables i.e. salt concentration and days after salt stress (A-E). These photographs had been published in the same journal from the same contributors in, two versions as described below; Versions of this article developed as 1 no. panel was made available when the article was “in press” and published online on 17-05-2010  and 2 no. panel was…

  • Fabrication,  Image Duplication,  Plagiarism

    Poor mouse.. again a culprit

    Share this...FacebookLinkedinGoogle+TwitterPrintemail From the earlier incidence, we followed the author and found that it’s not a single case of image duplication in an experiment to treat diabetes. Another study published in Nature Scientific Reports also have the clear effects of image duplication. Again the mouse has been utilized as a system to investigate the effect of various diabetes modulating drugs but tested has been conducted on the same mouse again and again. This image also has the repetitive image from Killing Diabetes or Mouse which will be followed up in next post.