• Fabrication,  Image Duplication,  Plagiarism,  Quality research

    A detailed story of the moving larvae …

    Share this...FacebookLinkedinGoogle+TwitterPrintemailIn our earlier post, we found that same larvae has been planned to move for another photograph. close examination of the photographs reveals that only a single photograph has been taken and second photograph has been used as cropped version. We mailed author  to explain this incidence, but did not get any replies. Hence we are providing a link to the article. http://10.1016/j.jinsphys.2008.12.005 In the article, author aimed to Silence acetylcholinesterase gene of Helicoverpa armigera by feeding siRNA supplemented in an artificial diet and reports that siRNA affects the larval growth and its life cycle. As we mentioned earlier, first photograph is to check the lethal dose of AChE-siRNA on larval growth. …

  • Image Duplication,  Quality research,  Science Education

    Larvae moving together for a photoshoot for another experiment

    Share this...FacebookLinkedinGoogle+TwitterPrintemailIf a researcher is doing some experiments with animal species, it is quite obvious that animal tends to move from one place to other. Movement of any animal species in a laboratory is difficult to manage. For this, we have to screw up the caps of culture tubes, put the tightened cotton plugs, clean the floors routinely and put an animal back if it comes out of its boundaries. Below we are discussing the possibilities, how a pair of larvae moved out of an experimental set to get a photoshoot for another set of conditions. Molecular biologist treats larvae with siRNA to check effect as lethality or as growth…

  • Quality research,  Science Education

    An author should explain more about the relevant experimental details

    Share this...FacebookLinkedinGoogle+TwitterPrintemailFrom a research article, we assume that author made everything clear and one could follow the research easily. This is the only reason, we made our research publicly available. But there are articles which led to the confusion to the researchers. The below-discussed article is among those ones. Here author used a gene to transform a model plant, but; • No cloning strategy (restriction sites, size) has been given for the gene;• Growth media compositions (YEM, root medium and other tissue culture media) are given in fine details (with elemental composition) for a model plant, unnecessarily.• For transgenics confirmation, both NPTII and gene-specific primer sequence and annealing temperature (Ta) is given…

  • Fabrication,  Quality research,  Science Education

    Southern or Photoshop! Use any for Transgenics Confirmation

    Share this...FacebookLinkedinGoogle+TwitterPrintemailWith Photoshop, we can do a lot of experimentation, like done here. Molecular characterization of transgenics is done to check the stable integration of the T-DNA cassettes. In two back to back papers authors did the experimentation to prove that transgenics are stable and contain the T-DNA sequence.  Two important sights are: Whenever we run an Agarose gel all the lanes are likely separated by equal distances, because of similar gaps between the comb teeth. However, in these pictures of the Southern blots, the gap between lanes is unequal, which is noticeable. This unequal distance led us to carefully investigate for the bands. Upon examination, we found that bands are created…

  • Quality research,  Science Education

    Does Over-expression of a gene leads to gene loss in rice?

    Share this...FacebookLinkedinGoogle+TwitterPrintemailBelow given picture is to confirm the presence of transgenes in rice transgenics. For over-expression studies, transgenics have been developed using GLYI gene of Brassica and GLYII gene of Rice. Upon transformation, it is obvious that WT Rice plants (controls) would show up a signal for presence of genomic GLYII gene. This is because, gene has been cloned from Rice and highly conserved among rice species. However, author didn’t detect presence of even a faint band in either PCR or Southern experiments. What could be the possible reason? We can discuss here. Full length article can be inquired at admin@vigilantscientist.com 9

  • Quality research,  Science Education

    Doing experiments unnecessarily and mis-interpreting data

    Share this...FacebookLinkedinGoogle+TwitterPrintemailBelow given picture is from Southern experiments of Rice T0 and T1 transgnics for very famous CRY genes transformation. Analysis of transgenics Here an unnecessary experiments has been done which does not add any value to the “PNAS” paper and “Bose Institute” and a mis-interpretation of data is given which is purely bases on assumpions. Please check and discuss with your friends to search for the issues highlighted. 8

  • Fabrication,  Publishing Error,  Quality research

    Article in press! Figure Altered! Published Differently. Reason for Antar Dhundo..

    Share this...FacebookLinkedinGoogle+TwitterPrintemailYou might have enjoyed our post “Antar Dhundo“, as it contains two panels 1 and 2, which are almost similar with a few differences, which we asked you to identify. You will be amazed to know that two panels are for “Northern blots” of RNA isolated from different samples harvested under two variables i.e. salt concentration and days after salt stress (A-E). These photographs had been published in the same journal from the same contributors in, two versions as described below; Versions of this article developed as 1 no. panel was made available when the article was “in press” and published online on 17-05-2010  and 2 no. panel was…

  • Fabrication,  Mislabeled,  Patent Technology,  Quality research,  Science Education

    Does N-Terminal tag impound any impact on transgene expression enhancement? As claimed!

    Share this...FacebookLinkedinGoogle+TwitterPrintemailFor a patent, claim is adding N-terminal tag enhances protein expression. But the smear and other bands present in 2-4 no lanes indicate that in lane 1 less amount of over-expressed protein has been checked. If the N-tag is making a difference for the over-expressed protein then other proteins should remain same in all lanes.What do you interpret and think from such a SDS gel, which is submitted as a patentable technology?http://nabi.res.in/frontend/web/pdf/achievements/AnexpressionVector.pdf 2