Quality research,  Science Education

Does Over-expression of a gene leads to gene loss in rice?

Below given picture is to confirm the presence of transgenes in rice transgenics.

For over-expression studies, transgenics have been developed using GLYI gene of Brassica and GLYII gene of Rice.

Upon transformation, it is obvious that WT Rice plants (controls) would show up a signal for presence of genomic GLYII gene. This is because, gene has been cloned from Rice and highly conserved among rice species. However, author didn’t detect presence of even a faint band in either PCR or Southern experiments. What could be the possible reason? We can discuss here.

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