For over-expression studies, transgenics have been developed using GLYI gene of Brassica and GLYII gene of Rice.
Upon transformation, it is obvious that WT Rice plants (controls) would show up a signal for presence of genomic GLYII gene. This is because, gene has been cloned from Rice and highly conserved among rice species. However, author didn’t detect presence of even a faint band in either PCR or Southern experiments. What could be the possible reason? We can discuss here.
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