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Does N-Terminal tag impound any impact on transgene expression enhancement? As claimed!

For a patent, claim is adding N-terminal tag enhances protein expression. But the smear and other bands present in 2-4 no lanes indicate that in lane 1 less amount of over-expressed protein has been checked. 
If the N-tag is making a difference for the over-expressed protein then other proteins should remain same in all lanes.
What do you interpret and think from such a SDS gel, which is submitted as a patentable technology?http://nabi.res.in/frontend/web/pdf/achievements/AnexpressionVector.pdf


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