• Quality research,  Science Education

    An author should explain more about the relevant experimental details

    From a research article, we assume that author made everything clear and one could follow the research easily. This is the only reason, we made our research publicly available. But there are articles which led to the confusion to the researchers. The below-discussed article is among those ones. Here author used a gene to transform a model plant, but; • No cloning strategy (restriction sites, size) has been given for the gene;• Growth media compositions (YEM, root medium and other tissue culture media) are given in fine details (with elemental composition) for a model plant, unnecessarily.• For transgenics confirmation, both NPTII and gene-specific primer sequence and annealing temperature (Ta) is given in…

  • Fabrication,  Quality research,  Science Education

    Southern or Photoshop! Use any for Transgenics Confirmation

    With Photoshop, we can do a lot of experimentation, like done here. Molecular characterization of transgenics is done to check the stable integration of the T-DNA cassettes. In two back to back papers authors did the experimentation to prove that transgenics are stable and contain the T-DNA sequence.  Two important sights are: Whenever we run an Agarose gel all the lanes are likely separated by equal distances, because of similar gaps between the comb teeth. However, in these pictures of the Southern blots, the gap between lanes is unequal, which is noticeable. This unequal distance led us to carefully investigate for the bands. Upon examination, we found that bands are created there,…

  • Fabrication,  Image Duplication,  Plagiarism

    Twist, Flip, Shift; Ready to publish.

    Earlier we identified that gene is missing in a PCR reaction and Southern experiment of the Rice transgenics. This is the content of an article under DOI (Could be enquired through mail at admin@vigilantscientist.com) Interaction with authors revealed that, a critical piece of information (about cloning and restriction digestion strategy in pCAMBIA, primer sequences for PCR and cloning, complete Southern blot figure) is missing in the article. Keeping it aside, we found that article is not carrying a single labeling error, but contains a smartly fabricated photograph which is very unique and new to our posts. In same article, author gave two different stress treatments i.e. Salt and Methylglyoxal (MG)  to…

  • Fabrication,  Image Duplication,  Plagiarism

    One Picture; Many Papers i.e. JNU & ICGEB

    From the producer of our story Antar Dhundo, we found that there is not a single event for image manipulation in the article. The follow-up studies have the usage of the same photographs again and again. From the below-given panels (4 different panels) you can see how the same figures have been used in 3 different articles. Institutions of national reputation like JNU and ICGEB are enjoying huge funding to carry out these studies from DBT, DST, CSIR etc. http://10.1099/mic.0.019869-0http://10.1074/jbc.m110.111021http://10.4161/psb.6.5.15106 4

  • Quality research,  Science Education

    Does Over-expression of a gene leads to gene loss in rice?

    Below given picture is to confirm the presence of transgenes in rice transgenics. For over-expression studies, transgenics have been developed using GLYI gene of Brassica and GLYII gene of Rice. Upon transformation, it is obvious that WT Rice plants (controls) would show up a signal for presence of genomic GLYII gene. This is because, gene has been cloned from Rice and highly conserved among rice species. However, author didn’t detect presence of even a faint band in either PCR or Southern experiments. What could be the possible reason? We can discuss here. Full length article can be inquired at admin@vigilantscientist.com 9

  • Quality research,  Science Education

    Doing experiments unnecessarily and mis-interpreting data

    Below given picture is from Southern experiments of Rice T0 and T1 transgnics for very famous CRY genes transformation. Analysis of transgenics Here an unnecessary experiments has been done which does not add any value to the “PNAS” paper and “Bose Institute” and a mis-interpretation of data is given which is purely bases on assumpions. Please check and discuss with your friends to search for the issues highlighted. 8

  • Fabrication,  Image Duplication,  Plagiarism

    Samanta Dhundo…. Find for the similars

    Dear Friends, Today’s story is exactly opposite the Antar Dhundo story. Here we have to find for the similars. Find for the similars Each lane contain different samples of glycine max (either roots or leaves under salt stress condition) and a semi-q-RT-PCR reaction was performed using different pairs of primers corresponding to the genes highlighted in front of the rows. In this figure a few (3-4) lanes of 7 samples each are similar. Article is produced from India and China in collaboration. Let’s see how many you can highlight. 7

  • Plagiarism

    Author might have made the changes in band intensities.

    In this post, we are sharing the possible reason how the changes occured in the two photographs before and after publishing. Till the article was in press, article contained Panel 1. In Panel 1, similar colour square boxes are the clones of bands. Like, bands in Red are identical, Green are identical; and Blue are identical to each other. How this could happen that bands are cloned in a Northern blot? Only author knows better. At the time of publishing, press might have asked author, for a better quality of photograph, so as it comes up in printable media as shown on the computer screen. On this, author thought of,…

  • Fabrication,  Publishing Error,  Quality research

    Article in press! Figure Altered! Published Differently. Reason for Antar Dhundo..

    You might have enjoyed our post “Antar Dhundo“, as it contains two panels 1 and 2, which are almost similar with a few differences, which we asked you to identify. You will be amazed to know that two panels are for “Northern blots” of RNA isolated from different samples harvested under two variables i.e. salt concentration and days after salt stress (A-E). These photographs had been published in the same journal from the same contributors in, two versions as described below; Versions of this article developed as 1 no. panel was made available when the article was “in press” and published online on 17-05-2010  and 2 no. panel was available…