• Fabrication

    Clear your Bands……

    Share this...FacebookLinkedinTwitterPrintemailThis is in continuation with our earlier post i.e. Real Bands. Below is the clarification of how author tilted the gel and used the wrtong hybridization signals to represent small RNA bands. Below are the reasons these should not be considered as bands. Bands always have a sharp edge on both sides. Bands appeared bearing a trail of smear

  • Fabrication,  Image Duplication

    In a gel, few well chose to double themselves. Why?

    Share this...FacebookLinkedinTwitterPrintemailAfter a long vacation of Deepawali, we are with a very new gel picture, where author fabricated the gel lanes. From the gel, it is clear that the picture was available for 38 samples (1-38) and after run completion, it has been decided to make a copy of the selected lane (17-21) and paste it as different samples (39-43).   We sent the data to the authors for moderation and article link will be updated soon. Please share your comments on this data. 2

  • Image Duplication,  Quality research,  Science Education

    Larvae moving together for a photoshoot for another experiment

    Share this...FacebookLinkedinTwitterPrintemailIf a researcher is doing some experiments with animal species, it is quite obvious that animal tends to move from one place to other. Movement of any animal species in a laboratory is difficult to manage. For this, we have to screw up the caps of culture tubes, put the tightened cotton plugs, clean the floors routinely and put an animal back if it comes out of its boundaries. Below we are discussing the possibilities, how a pair of larvae moved out of an experimental set to get a photoshoot for another set of conditions. Molecular biologist treats larvae with siRNA to check effect as lethality or as growth…

  • Quality research,  Science Education

    An author should explain more about the relevant experimental details

    Share this...FacebookLinkedinTwitterPrintemailFrom a research article, we assume that author made everything clear and one could follow the research easily. This is the only reason, we made our research publicly available. But there are articles which led to the confusion to the researchers. The below-discussed article is among those ones. Here author used a gene to transform a model plant, but; • No cloning strategy (restriction sites, size) has been given for the gene;• Growth media compositions (YEM, root medium and other tissue culture media) are given in fine details (with elemental composition) for a model plant, unnecessarily.• For transgenics confirmation, both NPTII and gene-specific primer sequence and annealing temperature (Ta) is given…

  • Fabrication,  Quality research,  Science Education

    Southern or Photoshop! Use any for Transgenics Confirmation

    Share this...FacebookLinkedinTwitterPrintemailWith Photoshop, we can do a lot of experimentation, like done here. Molecular characterization of transgenics is done to check the stable integration of the T-DNA cassettes. In two back to back papers authors did the experimentation to prove that transgenics are stable and contain the T-DNA sequence.  Two important sights are: Whenever we run an Agarose gel all the lanes are likely separated by equal distances, because of similar gaps between the comb teeth. However, in these pictures of the Southern blots, the gap between lanes is unequal, which is noticeable.  This unequal distance led us to carefully investigate for the bands. Upon examination, we found that bands are…

  • Fabrication,  Image Duplication,  Plagiarism

    Twist, Flip, Shift; Ready to publish.

    Share this...FacebookLinkedinTwitterPrintemailEarlier we identified that gene is missing in a PCR reaction and Southern experiment of the Rice transgenics. This is the content of an article under DOI (Could be enquired through mail at admin@vigilantscientist.com) Interaction with authors revealed that, a critical piece of information (about cloning and restriction digestion strategy in pCAMBIA, primer sequences for PCR and cloning, complete Southern blot figure) is missing in the article. Keeping it aside, we found that article is not carrying a single labeling error, but contains a smartly fabricated photograph which is very unique and new to our posts. In same article, author gave two different stress treatments i.e. Salt and Methylglyoxal (MG)…

  • Fabrication,  Image Duplication,  Plagiarism

    One Picture; Many Papers i.e. JNU & ICGEB

    Share this...FacebookLinkedinTwitterPrintemailFrom the producer of our story Antar Dhundo, we found that there is not a single event for image manipulation in the article. The follow-up studies have the usage of the same photographs again and again. From the below-given panels (4 different panels) you can see how the same figures have been used in 3 different articles. Institutions of national reputation like JNU and ICGEB are enjoying huge funding to carry out these studies from DBT, DST, CSIR etc. http://10.1099/mic.0.019869-0 http://10.1074/jbc.m110.111021 http://10.4161/psb.6.5.15106 4

  • Fabrication,  Image Duplication,  Plagiarism

    Poor mouse.. again a culprit

    Share this...FacebookLinkedinTwitterPrintemailFrom the earlier incidence, we followed the author and found that it’s not a single case of image duplication in an experiment to treat diabetes. Another study published in Nature Scientific Reports also have the clear effects of image duplication. Again the mouse has been utilized as a system to investigate the effect of various diabetes modulating drugs but tested has been conducted on the same mouse again and again. This image also has the repetitive image from Killing Diabetes or Mouse which will be followed up in next post.

  • Fabrication,  Image Duplication

    Bacterial assays are moving …..

    Share this...FacebookLinkedinTwitterPrintemailIn an importance screening assay, the same bacterial species is serving as one at a time and as other, next time. This is from supplemental information which we usually not check if we are not into the experiment or not following the studies. Most of these studies are served as “stand-alone papers” and get citations by “citation cartel”. Please go through figure and comment in the box.

  • Fabrication,  Image Duplication

    Killing Diabetes or Mouse ?

    Share this...FacebookLinkedinTwitterPrintemailThe author used different formulations of silver nanoparticles and bamboo cellulose crystals to heal up the wound on mouse skin surface. In such an experiment, it is obligatory that different mouse should be used to treat with different formulations made. This is to ensure the impact of any formulation on the wound healing or combating with the disease. However, the author has chosen the same mouse again and again to check the effect of different formulations. Like Yellow and blue color enclosed photographs indicate the same mouse used to treat with different formulations i.e. UC, DH-CNCs, BB-CNCs treatment for one and Bet and AgNPs for another mouse. Similar is the case…