• Fabrication

    Clear your Bands……

    Share this...FacebookLinkedinTwitterPrintemailThis is in continuation with our earlier post i.e. Real Bands. Below is the clarification of how author tilted the gel and used the wrtong hybridization signals to represent small RNA bands. Below are the reasons these should not be considered as bands. Bands always have a sharp edge on both sides. Bands appeared bearing a trail of smear

  • Fabrication

    Real bands or really bands?

    Share this...FacebookLinkedinTwitterPrintemailIn continuation with the earlier posts (genetic/gel engineering and three errors) Northern Blots shown below represents the presence of small RNA in the insect gut after feeding on dsRNA molecules. The pattern of bands corresponding to 50 and 21 nt, observed in these blots is unique as author  played a trick which results in formation of these bands. Your contribution or explanation is needed on the possible ways by which these bands are made. This post is part of our ongoing quiz. Answers and winner name will be disclosed on 30th November 2018. Drop your answers as comments or by email at admin@vigilantscientist.com. 1

  • Fabrication,  Image Duplication

    Genetic/Gel Engineering

    Share this...FacebookLinkedinTwitterPrintemailBelow is a marvelous example of gel fabrication. You can call it “gel engineering” instead of “genetic engineering”. In the picture, three PCR reactions for three genes and corresponding gels has been provided. But author had smartly made the engineering of gels to create this example. We identified a minimum of 4 spots where engineering has been done in these gels. We want that you should identify these spots and send us the replies at info@vigilantscientist.com or as comments. This is from Dr Sujata M from IIOR, ICAR, Hyderabad, http://www.icar-iior.org.in/index.php/drmsujatha 2

  • Fabrication

    Molecular Markers-Its easy to make and hide fabricated gels in a crowd of lanes

    Share this...FacebookLinkedinTwitterPrintemailIn continuation, with yesterdays post we found articles from the same laboratory contain gels of molecular markers which are highly fabricated. Below is the gel picture from Plant Science Journal where SCoT molecular markers have been used to check the polymorphism among a germplasm. The experiment is to demarcate the toxic and non-toxic features. However, author smartly fabricated the gel after running to show the results. From the data, it is difficult to relate the findings to the toxic and non-toxic feature of plants. Hence the whole experimentation and article are of no use both to the science or society. These experiments are very harmful and their presence in database could mislead the followers.…

  • Fabrication,  Image Duplication

    In a gel, few well chose to double themselves. Why?

    Share this...FacebookLinkedinTwitterPrintemailAfter a long vacation of Deepawali, we are with a very new gel picture, where author fabricated the gel lanes. From the gel, it is clear that the picture was available for 38 samples (1-38) and after run completion, it has been decided to make a copy of the selected lane (17-21) and paste it as different samples (39-43).   We sent the data to the authors for moderation however, no replies hav been received. This is from Dr Sujata M from IIOR, ICAR, Hyderabad, http://www.icar-iior.org.in/index.php/drmsujatha 3

  • Quality research,  Science Education

    An author should explain more about the relevant experimental details

    Share this...FacebookLinkedinTwitterPrintemailFrom a research article, we assume that author made everything clear and one could follow the research easily. This is the only reason, we made our research publicly available. But there are articles which led to the confusion to the researchers. The below-discussed article is among those ones. Here author used a gene to transform a model plant, but; • No cloning strategy (restriction sites, size) has been given for the gene;• Growth media compositions (YEM, root medium and other tissue culture media) are given in fine details (with elemental composition) for a model plant, unnecessarily.• For transgenics confirmation, both NPTII and gene-specific primer sequence and annealing temperature (Ta) is given…

  • Fabrication,  Quality research,  Science Education

    Southern or Photoshop! Use any for Transgenics Confirmation

    Share this...FacebookLinkedinTwitterPrintemailWith Photoshop, we can do a lot of experimentation, like done here. Molecular characterization of transgenics is done to check the stable integration of the T-DNA cassettes. In two back to back papers authors did the experimentation to prove that transgenics are stable and contain the T-DNA sequence.  Two important sights are: Whenever we run an Agarose gel all the lanes are likely separated by equal distances, because of similar gaps between the comb teeth. However, in these pictures of the Southern blots, the gap between lanes is unequal, which is noticeable.  This unequal distance led us to carefully investigate for the bands. Upon examination, we found that bands are…

  • Fabrication,  Image Duplication,  Plagiarism

    Twist, Flip, Shift; Ready to publish.

    Share this...FacebookLinkedinTwitterPrintemailEarlier we identified that gene is missing in a PCR reaction and Southern experiment of the Rice transgenics. This is the content of an article under DOI (Could be enquired through mail at admin@vigilantscientist.com) Interaction with authors revealed that, a critical piece of information (about cloning and restriction digestion strategy in pCAMBIA, primer sequences for PCR and cloning, complete Southern blot figure) is missing in the article. Keeping it aside, we found that article is not carrying a single labeling error, but contains a smartly fabricated photograph which is very unique and new to our posts. In same article, author gave two different stress treatments i.e. Salt and Methylglyoxal (MG)…

  • Fabrication,  Image Duplication,  Plagiarism

    One Picture; Many Papers i.e. JNU & ICGEB

    Share this...FacebookLinkedinTwitterPrintemailFrom the producer of our story Antar Dhundo, we found that there is not a single event for image manipulation in the article. The follow-up studies have the usage of the same photographs again and again. From the below-given panels (4 different panels) you can see how the same figures have been used in 3 different articles. Institutions of national reputation like JNU and ICGEB are enjoying huge funding to carry out these studies from DBT, DST, CSIR etc. http://10.1099/mic.0.019869-0 http://10.1074/jbc.m110.111021 http://10.4161/psb.6.5.15106 4