• Quality research,  Science Education

    Does Over-expression of a gene leads to gene loss in rice?

    Share this...FacebookLinkedinTwitterPrintemailBelow given picture is to confirm the presence of transgenes in rice transgenics. For over-expression studies, transgenics have been developed using GLYI gene of Brassica and GLYII gene of Rice. Upon transformation, it is obvious that WT Rice plants (controls) would show up a signal for presence of genomic GLYII gene. This is because, gene has been cloned from Rice and highly conserved among rice species. However, author didn’t detect presence of even a faint band in either PCR or Southern experiments. What could be the possible reason? We can discuss here. Full length article can be inquired at admin@vigilantscientist.com 9

  • Fabrication,  Image Duplication,  Plagiarism

    Samanta Dhundo…. Find for the similars

    Share this...FacebookLinkedinTwitterPrintemailDear Friends, Today’s story is exactly opposite the Antar Dhundo story. Here we have to find for the similars. Each lane contain different samples of glycine max (either roots or leaves under salt stress condition) and a semi-q-RT-PCR reaction was performed using different pairs of primers corresponding to the genes highlighted in front of the rows. In this figure a few (3-4) lanes of 7 samples each are similar. Article is produced from India and China in collaboration. Let’s see how many you can highlight. 7

  • Plagiarism

    Author might have made the changes in band intensities.

    Share this...FacebookLinkedinTwitterPrintemailIn this post, we are sharing the possible reason how the changes occured in the two photographs before and after publishing. Till the article was in press, article contained Panel 1. In Panel 1, similar colour square boxes are the clones of bands. Like, bands in Red are identical, Green are identical; and Blue are identical to each other. How this could happen that bands are cloned in a Northern blot? Only author knows better. At the time of publishing, press might have asked author, for a better quality of photograph, so as it comes up in printable media as shown on the computer screen. On this, author thought…

  • Fabrication,  Image Duplication,  Plagiarism

    Poor mouse.. again a culprit

    Share this...FacebookLinkedinTwitterPrintemailFrom the earlier incidence, we followed the author and found that it’s not a single case of image duplication in an experiment to treat diabetes. Another study published in Nature Scientific Reports also have the clear effects of image duplication. Again the mouse has been utilized as a system to investigate the effect of various diabetes modulating drugs but tested has been conducted on the same mouse again and again. This image also has the repetitive image from Killing Diabetes or Mouse which will be followed up in next post.

  • Fabrication,  Image Duplication

    Bacterial assays are moving …..

    Share this...FacebookLinkedinTwitterPrintemailIn an importance screening assay, the same bacterial species is serving as one at a time and as other, next time. This is from supplemental information which we usually not check if we are not into the experiment or not following the studies. Most of these studies are served as “stand-alone papers” and get citations by “citation cartel”. Please go through figure and comment in the box.

  • Honest error

    Bands are feeling very crowded..Space not available for others

    Share this...FacebookLinkedinTwitterPrintemailOur first post on western blotting. Here we observed that there is not enough space available to accommodate 2 more bands (in lane 1 and 3), if they were present after IPTG induction. Because, if we consider, that bands appeared in lane no. 2 and 4 are normal than the space for lane 1 and 3 is not enough to accommodate any band. This is the published work from the same author for whom we published our earlier post Killing Diabetes or mouse. Share your views.. https://www.sciencedirect.com/science/article/pii/S0141813017305664?via%3Dihub

  • Fabrication,  Mislabeled,  Patent Technology,  Quality research,  Science Education

    Does N-Terminal tag impound any impact on transgene expression enhancement? As claimed!

    Share this...FacebookLinkedinTwitterPrintemailFor a patent, claim is adding N-terminal tag enhances protein expression. But the smear and other bands present in 2-4 no lanes indicate that in lane 1 less amount of over-expressed protein has been checked. If the N-tag is making a difference for the over-expressed protein then other proteins should remain same in all lanes.What do you interpret and think from such a SDS gel, which is submitted as a patentable technology?http://nabi.res.in/frontend/web/pdf/achievements/AnexpressionVector.pdf 3