• Fabrication

    Real bands or really bands?

    Share this...FacebookLinkedinTwitterPrintemailIn continuation with the earlier posts (genetic/gel engineering and three errors) Northern Blots shown below represents the presence of small RNA in the insect gut after feeding on dsRNA molecules. The pattern of bands corresponding to 50 and 21 nt, observed in these blots is unique as author  played a trick which results in formation of these bands. Your contribution or explanation is needed on the possible ways by which these bands are made. This post is part of our ongoing quiz. Answers and winner name will be disclosed on 30th November 2018. Drop your answers as comments or by email at admin@vigilantscientist.com. 1

  • Fabrication,  Image Duplication

    Genetic/Gel Engineering

    Share this...FacebookLinkedinTwitterPrintemailBelow is a marvelous example of gel fabrication. You can call it “gel engineering” instead of “genetic engineering”. In the picture, three PCR reactions for three genes and corresponding gels has been provided. But author had smartly made the engineering of gels to create this example. We identified a minimum of 4 spots where engineering has been done in these gels. We want that you should identify these spots and send us the replies at info@vigilantscientist.com or as comments. This is from Dr Sujata M from IIOR, ICAR, Hyderabad, http://www.icar-iior.org.in/index.php/drmsujatha 2

  • Fabrication

    Molecular Markers-Its easy to make and hide fabricated gels in a crowd of lanes

    Share this...FacebookLinkedinTwitterPrintemailIn continuation, with yesterdays post we found articles from the same laboratory contain gels of molecular markers which are highly fabricated. Below is the gel picture from Plant Science Journal where SCoT molecular markers have been used to check the polymorphism among a germplasm. The experiment is to demarcate the toxic and non-toxic features. However, author smartly fabricated the gel after running to show the results. From the data, it is difficult to relate the findings to the toxic and non-toxic feature of plants. Hence the whole experimentation and article are of no use both to the science or society. These experiments are very harmful and their presence in database could mislead the followers.…

  • Fabrication,  Image Duplication

    In a gel, few well chose to double themselves. Why?

    Share this...FacebookLinkedinTwitterPrintemailAfter a long vacation of Deepawali, we are with a very new gel picture, where author fabricated the gel lanes. From the gel, it is clear that the picture was available for 38 samples (1-38) and after run completion, it has been decided to make a copy of the selected lane (17-21) and paste it as different samples (39-43).   We sent the data to the authors for moderation however, no replies hav been received. This is from Dr Sujata M from IIOR, ICAR, Hyderabad, http://www.icar-iior.org.in/index.php/drmsujatha 3

  • Fabrication,  Image Duplication,  Plagiarism,  Quality research

    A detailed story of the moving larvae …

    Share this...FacebookLinkedinTwitterPrintemailIn our earlier post, we found that same larvae has been planned to move for another photograph. close examination of the photographs reveals that only a single photograph has been taken and second photograph has been used as cropped version. We mailed author  to explain this incidence, but did not get any replies. Hence we are providing a link to the article. http://10.1016/j.jinsphys.2008.12.005 In the article, author aimed to Silence acetylcholinesterase gene of Helicoverpa armigera by feeding siRNA supplemented in an artificial diet and reports that siRNA affects the larval growth and its life cycle. As we mentioned earlier, first photograph is to check the lethal dose of AChE-siRNA on larval growth. …

  • Quality research,  Science Education

    An author should explain more about the relevant experimental details

    Share this...FacebookLinkedinTwitterPrintemailFrom a research article, we assume that author made everything clear and one could follow the research easily. This is the only reason, we made our research publicly available. But there are articles which led to the confusion to the researchers. The below-discussed article is among those ones. Here author used a gene to transform a model plant, but; • No cloning strategy (restriction sites, size) has been given for the gene;• Growth media compositions (YEM, root medium and other tissue culture media) are given in fine details (with elemental composition) for a model plant, unnecessarily.• For transgenics confirmation, both NPTII and gene-specific primer sequence and annealing temperature (Ta) is given…

  • Fabrication,  Quality research,  Science Education

    Southern or Photoshop! Use any for Transgenics Confirmation

    Share this...FacebookLinkedinTwitterPrintemailWith Photoshop, we can do a lot of experimentation, like done here. Molecular characterization of transgenics is done to check the stable integration of the T-DNA cassettes. In two back to back papers authors did the experimentation to prove that transgenics are stable and contain the T-DNA sequence.  Two important sights are: Whenever we run an Agarose gel all the lanes are likely separated by equal distances, because of similar gaps between the comb teeth. However, in these pictures of the Southern blots, the gap between lanes is unequal, which is noticeable.  This unequal distance led us to carefully investigate for the bands. Upon examination, we found that bands are…

  • Fabrication,  Image Duplication,  Plagiarism

    One Picture; Many Papers i.e. JNU & ICGEB

    Share this...FacebookLinkedinTwitterPrintemailFrom the producer of our story Antar Dhundo, we found that there is not a single event for image manipulation in the article. The follow-up studies have the usage of the same photographs again and again. From the below-given panels (4 different panels) you can see how the same figures have been used in 3 different articles. Institutions of national reputation like JNU and ICGEB are enjoying huge funding to carry out these studies from DBT, DST, CSIR etc. http://10.1099/mic.0.019869-0 http://10.1074/jbc.m110.111021 http://10.4161/psb.6.5.15106 4

  • Plagiarism

    Author might have made the changes in band intensities.

    Share this...FacebookLinkedinTwitterPrintemailIn this post, we are sharing the possible reason how the changes occured in the two photographs before and after publishing. Till the article was in press, article contained Panel 1. In Panel 1, similar colour square boxes are the clones of bands. Like, bands in Red are identical, Green are identical; and Blue are identical to each other. How this could happen that bands are cloned in a Northern blot? Only author knows better. At the time of publishing, press might have asked author, for a better quality of photograph, so as it comes up in printable media as shown on the computer screen. On this, author thought…