• Fabrication,  Image Duplication

    Genetic/Gel Engineering

    Share this...FacebookLinkedinTwitterPrintemailBelow is a marvelous example of gel fabrication. You can call it “gel engineering” instead of “genetic engineering”. In the picture, three PCR reactions for three genes and corresponding gels has been provided. But author had smartly made the engineering of gels to create this example. We identified a minimum of 4 spots where engineering has been done in these gels. We want that you should identify these spots and send us the replies at info@vigilantscientist.com or as comments. This is from Dr Sujata M from IIOR, ICAR, Hyderabad, http://www.icar-iior.org.in/index.php/drmsujatha 2

  • Quality research,  Science Education

    An author should explain more about the relevant experimental details

    Share this...FacebookLinkedinTwitterPrintemailFrom a research article, we assume that author made everything clear and one could follow the research easily. This is the only reason, we made our research publicly available. But there are articles which led to the confusion to the researchers. The below-discussed article is among those ones. Here author used a gene to transform a model plant, but; • No cloning strategy (restriction sites, size) has been given for the gene;• Growth media compositions (YEM, root medium and other tissue culture media) are given in fine details (with elemental composition) for a model plant, unnecessarily.• For transgenics confirmation, both NPTII and gene-specific primer sequence and annealing temperature (Ta) is given…

  • Fabrication,  Image Duplication,  Plagiarism

    Twist, Flip, Shift; Ready to publish.

    Share this...FacebookLinkedinTwitterPrintemailEarlier we identified that gene is missing in a PCR reaction and Southern experiment of the Rice transgenics. This is the content of an article under DOI (Could be enquired through mail at admin@vigilantscientist.com) Interaction with authors revealed that, a critical piece of information (about cloning and restriction digestion strategy in pCAMBIA, primer sequences for PCR and cloning, complete Southern blot figure) is missing in the article. Keeping it aside, we found that article is not carrying a single labeling error, but contains a smartly fabricated photograph which is very unique and new to our posts. In same article, author gave two different stress treatments i.e. Salt and Methylglyoxal (MG)…

  • Quality research,  Science Education

    Doing experiments unnecessarily and mis-interpreting data

    Share this...FacebookLinkedinTwitterPrintemailBelow given picture is from Southern experiments of Rice T0 and T1 transgnics for very famous CRY genes transformation. Here an unnecessary experiments has been done which does not add any value to the “PNAS” paper and “Bose Institute” and a mis-interpretation of data is given which is purely bases on assumpions. Please check and discuss with your friends to search for the issues highlighted. 8